First, keep an eye on your water parameters just to make sure you broke through your cycle. Next, if you do use bio spira, (which I have done and I believe it works) then you can simulate fish for a few days just to make sure that the cycle really occured. What I mean is, you can add about 4 drops of pure ammonia from the local grocery store to the water and see if there are bacteria to eat it up. IF you test about 5 minutes after dosing the tank you should have high ammonia. IF you test the next day and your ammonia has dropped to almost nothing, I would say you have a good bacteria base formed.
39 gallon (2yrs) 40lbs live sand. 23 lbs LR
(still collecting) , 1 turbo snail, 5 Nasarius snails, 10 blue knuckle hermits, 1 zebra hermit, 1 scarlet hermit, 5 red tip hermits, 1 Green Chromis, 1 Blue Damsel, ? Ricordia, candy cane coral.
135gallon (2 months)
125lbs live sand, 100lbs live rock, 2 175W Metal Halide, 1 175W VHO Actinic (so far). Berlin Protein Skimmer in 40 Gallon Sump. 2 Maxi Jet 1200's. 10 Nassarius, 10 mexican turbos, 10 astreas, 20 Blue Leg Hermits, 20 Scarlet Hermits, 5 Emerald crabs, 5 peppermint shrimp. 1 Copper Band butterfly, 2 False Perculas, 1 Javanese Damsel.