Well it won't hurt it, but your prefilter and biomax will get dirty. I've been running FX5's (3) for a couple years and did remove the sponges once just to see and I was not happy with how coated the biomax got (and I did use the fluval prefilter media). So yes it can be done, it can make the flow alittle better than when the sponges get dirty and the filter is running, but the trade off to me just wasn't worth it. It's not a matter of how many/what kind of other filters you use, you still need mechnical filtration to keep your biological filtration from becoming overly dirty.