I partially disagree with some of the answers to this question thus far.
Do you still need to use dechlorinator? Absolutely!
Do you want to use Prime? Only with caution.
Any product that binds/locks-up/removes ammonia will hamper the effectiveness of Safe Start. Prime is one such product. If using Safe Start, it's best to use a dechlorinator only, and not one that also detoxifies ammonia like Prime.
In regards to ammonia products, yes, they kill TSS. Any type, whether a
chloramines remover or detoxifier, etc, anything that says it locks up
ammonia or removes ammonia. Do not add TSS for 24 hours after using
such a product, and do not add such a product for at least 7 days after
using TSS. The bacteria is housed in a special stabilized solution of
ammonia, so if you remove/lock up the ammonia, you remove all of the
food the bacteria require to live.
Source:
Q & A With Tetra about Tetra SafeStart | 58116
Best!