It's most likely a bacterial infection. Perhaps it became infected when she was put back in the tank. PArasites such as ich can leave tiny wounds behind and in a high nitrate system, can become easily infected. Nitrate limits should be no more than 40
ppm, but I even recommend lower than that. Especially with heavy waste producers. she's a big fish. She expells a lot of waste. Fish also expel waste (ammonia which eventually becomes nitrate) through their gills from breathing. Other than for size sake, another reason for a bigger tank is for more oxygenated water for good, clean respiration.
Stop feeds for about a week. She'll be fine. The important thing now is to get the nitrates under control so she can heal. High nitrate will hamper treatment efforts. There's a lot of bad bacteria in there.
I have found that a nitrate reducing routine of 10% water change with a vacuum two to three times a week. until nitrates reach an acceptable level has worked well with minimal stress on the fish. Try getting it down to 20 or even 30
ppm. Change carbon every two weeks as opposed to once a month while she's in that tank. NitraZorb pouches and DeNitrate pellets are excellent helpers in reducing nitrates along with the water changes. Feeding should only be about 3 times a week...no more than what she can consume in two minutes per day. I'd change the type of food too. Pellets can weigh heavy in the gravel and add to the nitrate problem. Picky eaters always seem to take a liking to thawed out frozen foods such as krill, silversides, beef heart, brine shrimp and a spiralina enrich food or algae based food. Add a bit of garlic in there about once a week or bi weekly to help prevent any external parasites from taking hold of her. It's also an appetite stimilant.
Medicating can be difficult while trying to reduce nitrates because of the water change routine. What I would do is go as directed on the medicine bottle for up to two weeks, then go to the above described routine for two weeks. If needed, retreat with a different medication.
Start with the methylene blue and malachite green treatment first. Then go on to the frequent small water changes for two weeks. IF nitrates are still high after those two weeks (anything reading above 40
ppm) continue for one more week. After, treat with the ampicilin for two weeks if the infection is still present. You can still get that??? Is the package new or is it something you've had on hand for awhile? It's hard to get here. It use to be always available but because of increased prices on penicillin, it's not economical anymore.
Get that 55 going soon. She needs the water volume just as much as the space
Also, it'd be a good idea to get a large bottle of Cycle or Nitromax...or even Mark Weiss's Bacter vital...a bio additive to dose in the tank. Go ahead and double dose after each water change. You can even pour it right onto the bio wheels. This will help prevent nitrite and ammonia from backing up and help with any ammonia spikes if any.
Another thing I thought of was to condition the tank with NovAqua water conditioner...that specific brand. It will help keep electrolites from escaping and will help aid in healing. It will also provide an extra coat of slime to prevent more bacteria from entering the wounds.
Good luck
Don't be surprised if the nitrates take a few months to get down. After the second treatment if it gets that far, start up the routine until nitrates are below 40ppm if it's still a bit high.
Hope I didn't confuse ya...lol. Ask if you have other questions.