sand substate, in general, is easier to maintain then CC. It is easily sifted by critters and does not collect detritus that lead to NO3. Technically, a true DSB is 5-6 inches. The theory is to provide an anaerobic environment for denitrifying bacterial to colonize, hence the depth. The problem is that pockets of toxic gas can also collect in the sand bed and, when released, can foul the tank. 2-3 inches is just fine when combined with the right amount of LR.