Hi, you would probably need around 2 bottles of DIY co2 for the tank.(could be wrong about that) but one bottle wont suffice for sure. All glass diffusers need a specific pressure for co2 to be released from them(learned this the hard way haha) you could try a small piece of chopstick shoved in the airline tubing. I haven't tried this my self but know people who have. Hope that helped!