I only found one good thread on this at aquariacentral and an article written by some guy, and they were both quite successful at getting the cycle to go through to nitrogen gas. Several people in the AC thread also said that they had luck in getting it to work.There was a thread a few months back where someone researched anoxic zones in sand and determined that a few inches of sand may not be deep enough to establish much if any anoxic zones. The worry was that while mixing up the sand during a gravel vac ... some anoxic gases could be released into the water and kill the stock.
It is an interesting idea if it can be done in an aquarium ... and if 3 inches of sand's deep enough. I would think you'd need really fine sand like play sand ... PFS is probably too coarse and would allow more water flow.
Waiting to read about experiences from members ....
I only found one good thread on this at aquariacentral and an article written by some guy, and they were both quite successful at getting the cycle to go through to nitrogen gas. Several people in the AC thread also said that they had luck in getting it to work.
As for the toxic gas, the idea is that you don't have to vac it because the detritus will settle on the sand and get worked in by MTS and blackworms, which can rot and feed the bacteria in the anaerobic zone. And if you do have to vac the sand, don't mix it up and vac an inch away so it can suck up the stuff sitting on the sand without moving sand.