Most people at loaches online seem to be open to the idea. I will acclimatize them 1st in a quarantine that begins with tap water and gradually increase the hardness to that of the main tank. It will probably be a 3 wk process. During that time they will be heat and salt treated for ick. Or, I could put them in the tank as soon as it is cycled and let them be the first inhabitants. Then I could treat them in there and gradually bring the hardness up.