Ashes2ashes
Aquarium Advice Regular
Ammo levels must be kept under .25ppm no mater how many water changes it takes. Almost none of the beneficial bacteria is in the water column. Back-to-back water changes, multiples in one day, can sometimes be necessary
to keep ammo levels low enough if you are cycling with fish, not too mention nitrite which needs to kept as close to 0 as possible.
Yep! With fish in cycling, water changes are your friend. It is neccessary to keep the ammo and nitrite low as to not damage the fish. Most of the beneficial bacteria lives in the filter and some in the gravel. and other surfaces, but like Siva said, very very little in the water column.