How stressed does a fish have to be before it dies?
Cortisol is an indicator of stress. It is found in the blood and it is released as a response to stress).
If the stress is persistent and of sufficient intensity, changes in the cellular structure of the gills may occur under the influence of cortisol. In this situation, increased death and turnover rates of branchial epithelial cells leads to accelerated aging of the gills. These degenerating and newly formed gill cells do not function normally, which further limits the fish’s ability to maintain water and ion homeostasis under stressful conditions. Thus, acute stress limits the fish’s capacity to osmoregulate, and prolonged periods of extreme stress may result in osmotic shock and death. (Joe Gargas Aquatics magazine 2011)
Rapid breathing is a sure sign that the fish is stressed. Erratic movement and twitching etc.
Rapid dissolved solid fluctuations (depending on ions involved ) caused acute and/or chronic stress.
Bullying causes stress and cortisol release and will likely show through with rapid breathing.
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