Clove oil is used to anaesthetize fish and is fatal to fish at doses greater than ¼ ml per litre of water. Clove oil is used in beer brewing and can be purchased through home-brewing outlets. It is also an aromatherapy oil, so try specialty outlets where oils are sold. Keep in mind that clove oil is classified as hazardous and can be irritating to the skin and eyes, and can be harmful if swallowed.
Since fish are cold-blooded, the brain can continue to function for a long time even after the heart and lungs have ceased functioning. Therefore it is possible for fish to recover from deep anaesthesia even if they are apparently dead because they are not breathing and have no heart beat. It can be very difficult to determine if a fish is dead, therefore, once the fish is deeply anaesthetized by leaving it in the solution for a couple of hours, it is recommended to freeze the fish, decapitate it or administer a sharp blow to the head to ensure it does not recover from anaesthesia.
Dr Rebecca Bragg BVSc MACVS