Originally Posted by neilanh
Sorry everyone, I didn't mean to stir up such a fuss with what I thought was a simple question.
Thanks for the great information Tom. I could see from all of the beautiful tanks on here that the pH swings obviously didn't bother the fish, I was hoping to understand the workings behind that - since many many places stress to newbies about the importance of a stable pH when keeping fish. I think I see where it all plays in now.
swings on fish health(osmoregulation)= bad.
swings(no osmoregulation)= not bad
No need to apologize, no one did anything wrong here.
Some took things a bit personal. They will get over it.
Simple questions are the best type actually, when the assumptions are challenged and considered well, then they can become pretty interesting.
is a deep well of assumptions and the impacts are not nearly as simplistic as I thought say 10 years ago.
Today I see that we know much less than we thought, and are just really seeing how our assumptions got us into trouble in the past.
I'm not interested in grilling anyone, I think many assume that about me.
I am interested in trying to learn more and help hobbyists learn more, how to set a test up, what observations look reasonable, which do and which do not match with our question, how we might rule one thing out as factor etc.
If some suggests an idea that has a bad assumption, "like all my test kits must be accurate", I'm going to get out the BBQ tongs and grill, but the goal is not to discredit anyone, never was. The idea and how it is applied are the issues.
It's to attack that issue, that idea, that suggest our test kits are great and accurate. We do not know unless we do something to make sure the test kit is accurately measuring a NO3
You need a reference and a control to compare something against.
If an expert suggests that we just brush it off, claims it;s no big deal etc I think calling them on it is very appropriate.
It's a bad method and they made an assumption, one that can and should be addressed.
A lot of advice is very general on the web, as such, it is not as precise for situations such as CO2
Galen just went with the best that Galen knew was all.
Galen offered help and cared enough to do so
Galen got irritated with my response, took it personal, no big deal.
Galen will get over it and hopefully see it is not a personal attack. Main issue is to think about how the pH affects our tanks in the context of CO2
enrichment and why it would/would not impact fish health.
Generally, in aquatic toxicity test, we use some biomarkers (stress proteins HSP 70's, mRNA, gonads etc) and some organisms: a plant, an invert critter and a fish. Then we do acute testing for a LD 50 dose response curve in the lab, then see how well this can be mimic in the field etc.
Long term or genetic issues can be addressed by looking at the F1, F2 populations, or measuring reproduction generally.
It's a very interesting field and you can help the environment and answer some cool questions. They also pay you