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Either after or during refilling. I do it afterwards. I've had African, and South American cichlids, for over thirty years. It won't hurt them for a few minutes. Unless you have a very large tank you should be able to fill it up very fast with a python.
I have an Oscar that is 16 years old and a Jack Dempsey that is a little less. I bought them within a few months of each other and they've survived numerous moves. The Red Devil I also bought with them just died about six months ago.
I have several saltwater fish over ten years old.
Beerman says:
Don't wash it. You'll lose the small, valuable particles.
Add liverock before the cycle, not after, or it will cycle again.
6" of sand for maximum dsb benefit.
IMO that's too many fish. I would cut it down to less then half that. I would also make salt. Too much risk of impurities since it won't dilute like the ocean.
I've gone a week several times between feedings for South American Cichlids because of work. Two weeks is pushing it. They'll live but will be pretty pissed at you when you return.