I haven't tried a great deal. Tested 3-4 times and it's been dark red each time. I have been changing 20% water every 1-2 weeks. I'm almost trying to prove denitrification is something that happens in a soil set up but I think time is not on my side now. Saying that, I've done copious amounts of research on nitrate toxicity and not surprisingly my fish show zero signs of distress but it is difficult to tell where exactly nitrates are at. Please don't follow my lead on nitrate toxicity and allow nitrates to go higher than the recommended levels, this tank is experimental and water will be changed immediately if any signs of distress occur. This would be nice if they came down on their own through denitrification but perhaps the substrate redox is too high to encourage denitrification which is a good thing of course.
Sent from my iPhone using Aquarium Advice