Glofish Breeding
Zebra danios are some of the easiest egglaying fish to breed in the aquarium, and glofish should be no different. If you have a pair of glowfish that spawns in the aquarium, their fry will also be glofish and not plain zebra danios. For regular zebra danios it is possible to tell the difference between males and females. Females have blue and silver stripes and males have blue and gold stripes, and the females usually have more of a rounded bodyshape. Other than body shape I'm not certain how you determine the sex of glofish.
During spawning they scatter their eggs. As with regular zebra danios, you should place marbles in the bottom of the breeding tank so that the parents won't be able to eat the eggs. You should also have plants in your spawning tank. After spawning is complete remove the parents from the tank and place them back into the regular aquarium.