The friendliest place on the web for anyone with an interest in aquariums or fish keeping!
If you have answers, please help by responding to the unanswered posts.
It doesn't absolutely have to be tank.water...I wash my filter floss in regular water, only because my hkb filter is strictly for detrius and hard waste, I don't need it for beneficial bacteria
On the aqueon quiet flow filters there is a "bio-holster" and the filter floss. They replaced the floss, so they should be fine, unless there is no bacteria in the holster.