There's a new twist to my trio. Today, just one of the females spawned with the male. HOWEVER, over the course of the past few days, I've watched the female that spawned attacking the other female just as she was doing prior to the trio spawn. The male just let them go at it for a while before stepping in and admonishing the aggressor. Today, however, he kept the second female from approaching the spawning site during the spawn. Since this is only a 15 gal tank, in theory, they should have really beaten her up if they didn't want her in there. Since there is a second spawning rock in the tank on the opposite side of the tank, the second female seems to be able to stay out of sight for now. Now, since they haven't really roughed her up too much, does this mean the Male will breed with the second female in the next few days ( when she should be more filled with eggs)? I hesitate to take out the second female in the hopes that this will happen. I also don't have a good matching male for her at the moment either.
I've taken to trying to hatch this spawn myself in a 2 1/2 gal tank using basically rainwater with a minimal amount of softened, PH regulated well water mixed in. I used Prime to make sure that there were no issues with any metals or organics in this new water. I've also used some M. Blue in the water as well. Tomorrow, the tank will be brought into the house for better climate control as it is still getting up into the 90s during the day so I want to eliminate that as the cause for all the dead eggs of the past.
My next step if this fails again, is to get a proven pair from my buddy and see what kind of results I get. Nothing says "Something's Wrong" like a proven pair of fish with dead eggs.
So as I've said before, Trios are a hard thing to keep going. It's just not natural for the fish. Thankfully, for now, I haven't lost the second female. I have a place to put her but nobody to pair her with. Time to go fish shopping again