Right sorry if this is blonde but when breaking in a tank and you get new tank syndrome wit really high ammonia? Well, isn't the growth of the denitrifying bacteria almost encouraged by the presence of ammonia in the water, and that's why they begin to grow? If so, instead of introducing fish into the water to supply the ammonia, couldn't you just well, wee into the tank? and as such wouldn't that help the tank 2 cycle? I was just thinking cos i am upgrading from a 70 litre tank to a 400 litre one. Thing is, I don't really want them running alongside each other for too long (space issues) and also I don't really want to jeapordise any fish that are put in initially (I think it's cruel). I know that you can get that stuff to help it cycle, but why not use what we throw away (that is, if it is suitable!). Doues anyone know if this would work or not?