Since you have a python you definitely want to keep the tubing clean. Those of us with simple gravel vacs that never use the tubing to go INTO the tank but rather just into a bucket can pretty much ignore the cleaning.
I would recommend using a bottle of hydrogen peroxide and pretending the bottle is your tank (to suck it through the tubing). If you can stop it in the tubing thats great, but even a quick shot through the tubing should help to sterilize it (even if it doesn't remove the specs it will kill them be it mold/bacteria/fungus/algae/etc.). Let it sit a minute or two. Then just rinse it out with tap water.
Bleach is another option but for obvious reasons I don't get that near established tanks with any equipment unless I clean it really well afterwards (just an extra hassle). Peroxide loses its effectiveness much more rapidly and is less of a problem on the off chance a small amount got into your tank.
HTH