It could be a build up of decaying organic material that was unable to breakdown because of the lack of o2. If you have a sand substrate you need to stir it up a bit every now and then. When I'm doing a PWC and vacuum I turn off the vac for a min and the filter as well and stir up a part of the sand. You don't want to do this too vigorously and kick up a ton of sand, but enough to turn it over a bit make sure to turn your filter off for a little while until and sand you kicked up settles bak down but you shouldn't have to leave it off too long. You just don't want the filter sucking up a bunch of sand because it can ruin your impeller or slow your filter flow down. Not hard after the first time you do it. Just start off with a small patch and watch out for an ammonia spike due to more organic material being broken down. You might have to do more frequent PWC.