I use the API (a.k.a Aquarium Pharmaceuticals) Master Freshwater Test Kit (be careful, they make a saltwater one too and that wont work at all for a freshwater tank).
It's a liquid titration kit (add tank water to line on vial - add drops - shake - check color) that comes with measures for PH, HighPH, Ammonia, Nitrite, and Nitrate.
The recommended way to start a fishless cycle is like this:
1. Take exactly 1 gallon of water, and treat it like you would for a water change.
2. Add a few drops of ammonia. Stir.
3. Check ammonia levels with test kit.
4. Repeat steps 2 and 3 until the 1 gallon of water gives a reading of 3-5ppm Ammonia.
5. Once your tank water is set up - simply multiply the number of drops it took to take the 1 gallon to 5ppm - and use that many to take the aquarium to 5ppm.
--Remember - when you set up a 45 gallon aquarium - that "45 gallons" is when it's completely empty - your tank will have quite a bit less than 45 gallons of actual water in it once you set up decorations and gravel/sand.--
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As for testing - here's the general regimen:
(Tank is dosed to 4ppm and filter is started)
(If you have lights for the tank - it's been said that it's better to leave them turned off all day during the cycle)
(If you have a heater, and you can turn it up to 83-85*F, that will accelerate the good bacteria)
1. Test for ammonia only, about once every 2 days.
2. When you notice the ammonia level dropping - do a test for ammonia and nitrite. Also - add enough drops of ammonia to "push it back to 4ppm" - you shouldn't have to test again after, as long as you did the math and added the correct # of drops.
3. Keep doing Step 2 every day, and you'll watch "Nitrites" get extremely high, and then start lowering. Remember to dose ammonia back to 4ppm when you can.
4. When you see "Nitrites" dropping - do tests for Ammo / Nitrite / Nitrate. Eventually you'll see both Ammonia and Nitrite at 0ppm - and Nitrates will be way off the chart.
You'll Know you're cycled when: A full dose of 4ppm of ammonia gets completely changed into "Nitrates" in under 24 hours.
Once that happens - do a huge water change (80% of the tank's water needs dumped) - and you're ready to bring your fish home.
If you finish cycling but won't get fish for awhile: Keep dosing some ammonia every morning to keep those bacteria rolling - and do another huge water change the day before you go get your fish.
That sounds like a very good plan, I think I can do that. Making a large instructional sheet and I think that will fill in the space for fishless cycling.
Just several questions I need to fill in.
1. Even though it's probably been said to me already, I just wanted to clarify, ALL things that are going in the tank go in during cycling, correct? Things like gravel, plants or rocks. This would technically help the spread of the good bacteria.
2. Only purchase fish after the tank itself is cycled?
3. Enlighten me on pre-main tank quarantine techniques. In my case, would I purchase several guppies and place them in a smaller 5-10 gallon tank first (for what reason, I don't know)? Or would I just buy a few at a time to see how they fair in the tank, then add more until the school is complete?
4. If pre-main tank quarantine is necessary, is a 5-10 gallon tank filled with dechlorinated water (no filters or anything) fine for the guppies for several days until they are deemed okay for the main tank?
5. Once each fish has made its rounds in the quarantine tank and the tank is cycled, is the tank officially set up?
6. When cleaning the filter, you remove it and swish it around water taken from the tank until the gunk is off, then you simply reattach it. Do you just replace the water taken from the tank with more dechlorinated water? It seems like this could also be used as the weekly 10-15% water change, but I won't clean the filter weekly simply to preserve the good bacteria.