I have a heavily planted tank and yes, use liquid CO2 every other day, as I have for the last 6-7 months. I haven't used it in a few days, and I am still fishing dead ones out, today there were two more. I have gravel, which I have always had. I can't stress enough how much and carefully I take of the two tanks. There are two bubblers on fine bubbles and the a fast moving filter. Thank you for bringing you thoughts to this. I am still ... mystified. My ten is now empty, and I will change the substrate, everything in the filter, except the ceramic rings which will be washed out with WCd water from the 20g because it seems that it has become stable. After I get the 10 stable then I will tear the 20 apart. There are fish missing and I can't find them, so it needs to be cleaned. I am sorry, I am laying my thoughts on you and complaining. Didn't mean to. Thank you for your thoughts and help!
Is your tank planted or do you dose any Co2? What kind of substrate do you have, sand, gravel, etc?
The fact hat it seems to be happening overnight could suggest a possible PH swing, but that is usually only associated with planted tanks. The plants take all the oxygen in the water, and the fish basically suffocate to death after the lights go out.
If you have a fine substrate, such as sand, perhaps there are some buildups of gas that are escaping into the tank.
I may just be grasping at straws for you....the only other things I can think of is some sort of contamination, or a quick killing disease.
I am so sorry for your loss, I would be devastated to find my tank desimated like that. Keeping my fingers crossed for you and your remaining finned friends.
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