Brooks, I literally just read the same FAQ that you likely did on the SeaChem website regarding testing while using Prime. I feel like I have a handle on it. That message was for Hubert. He suggested that I carelessly cycled with the Clown's in the tank and was oblivious to Ammonia and nitrite readings as a result and this is why the Clown's died. I take offense to that. Because I sat on that tank like a mother hen for over 3 weeks. What I read on the Seachem site is the test will make false POSITIVES. Meaning the test will show an ammonia level HIGHER than actual... not lower like he suggested. I used to see how many posts people had before reading their 'suggestions' but to be honest, I see that frequent posting does not an expert make!